Anti-PTPN22; clone 3B3.1

Application

Research CategorySignaling

This Anti-PTPN22 Antibody, clone 3B3.1 is validated for use in Western Blotting for the detection of PTPN22.

Research Sub CategoryDevelopmental Signaling

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Tyrosine-protein phosphatase non-receptor type 22 (EC:3.1.3.48; UniProt Q9Y2R2; also known as Hematopoietic cell protein-tyrosine phosphatase 70Z-PEP, Lymphoid phosphatase, Lymphoid-specific protein tyrosine phosphatase, Lyp, PEP, PEST-domain phosphatase, Protein tyrosine phosphatase non-receptor type 8) is encoded by the PTPN22 (also known as LYP, LYP1, LYP2, PTPN8) gene (Gene ID 26191) in human. PTPN22/Lyp is expressed in all hematopoietic cells and belongs to the PEST group of nonreceptor classical class I protein tyrosine phosphatases (PTPs). Lyp is a negative regulator of TCR signal transduction by targeting phosphorylated Lck (Y394), Fyn (Y427), and Zap-70, as well as phospho-sites on TCRζ, CD3ε, Vav, and Vcp. Lyp utilizes its proline-rich region 1 to interact with the SH3 domain of C-terminal Src kinase (Csk), resulting in enhanced inhibitory tuning of Src family kinases and TCR signal transduction. TRAF3 is also shown to bind the proline-rich region 1 of Lyp in myeloid cells and impact its turnover. Additional binding partners of Lyp include the adaptor molecule Grb2, the E3 ligase c-Cbl.

Immunogen

GST-tagged recombinant protein corresponding to the C-terminal of human PTPN22.

Epitope: C-terminal

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Mouse monoclonal IgG1κ in supernatant without preservatives.

Unpurified

Quality

Evaluated by Western Blotting in human burkitt′s lymphoma Ramos cell lysate.

Western Blotting Analysis: A 1:2,000 dilution of this antibody detected PTPN22 in 10 µg of human burkitt′s lymphoma Ramos cell lysate.

Specificity

Clone 3B3.1 is expected to react with spliced isoform 1 (LyP1), isoform 3, and isoform 4 (LyP3), but not isoform 2 (LyP2) or isoform 5. Reactivity toward isoform 6 (PTPN22.6) has not been tested.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Target description

~92 kDa observed. 91.7 kDa (isoform 1; Lyp1), 85.1 kDa (isoform 3), 88.6 kDa (isoform 4; Lyp3) calculated. Uncharacterized band(s) may appear in some lysates.